Quality
? Mouse colonies are routinely screened for pathogens via Mouse Antibody Production (MAP) testing
? Extensive PCR testing is performed to screen for a number of pathogens, including LDEV, to ensure strict control of raw materials used during the manufacturing process
? Tested and found negative for bacteria, fungi, and mycoplasma
? Protein concentrations are determined by Lowry method
? Endotoxin units are measured by Limulus Amoebocyte Lysate assay
? BD Matrigel Matrix gel stability is tested for a period of 14 days at 37°C
? Biological activity is determined for each lot using a neurite outgrowth assay. Chick dorsal root ganglia are plated on a 1.0 mm layer of BD Matrigel Matrix and must generate positive neurite outgrowth response after 48 hours without addition of nerve growth factor

Source
Engelbreth-Holm-Swarm Mouse Tumor

Preparation and Storage
Store at -20°C. Avoid multiple freeze-thaws. Do not store in frost-free freezer. KEEP FROZEN.

Application
? Cell Growth and Differentiation
? Metabolism/Toxicology Studies
? Invasion Assays
? In Vitro and In Vivo Angiogenesis Assays
? In Vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice